In initial molecular phylogenetic studies of protists, the 18S rRNA gene was used as a source of the DNA markers. But mainly due to low phylogenetic resolutions at the genus and species levels, 18S rRNA has been replaced by 28S rRNA. However, the limited availability of 28S rRNA sequence data prevents the use of protein-coding genes in various molecular comparisons. Eukaryotic Microbial 18S Sequencing https://www.microbialtec.com/eukaryotic-microbial-18s-sequencing.html
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Eukaryotic Microbial 18S Sequencing

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In initial molecular phylogenetic studies of protists, the 18S rRNA gene was used as a source of the DNA markers. But mainly due to low phylogenetic resolutions at the genus and species levels, 18S rRNA has been replaced by 28S rRNA. However, the limited availability of 28S rRNA sequence data prevents the use of protein-coding genes in various molecular comparisons. Eukaryotic Microbial 18S Sequencing https://www.microbialtec.com/eukaryotic-microbial-18s-sequencing.html